|
KMID : 0545120090190111374
|
|
Journal of Microbiology and Biotechnology
2009 Volume.19 No. 11 p.1374 ~ p.1378
|
|
|
Optimization of Capsular Polysaccharide Production by Streptococcus pneumoniae Type 3
|
|
Jin Sheng-De
Kim Young-Min
Kang Hee-Kyoung
Jung Seung-Jin
Kim Do-Man
|
|
|
Abstract
|
|
|
|
Response surface methodology (RSM) examining the effects of five-level-three-factors and their mutual interactions was utilized to optimize the fermentation conditions to enhance capsular polysaccharide (CPS) production of Streptococcus pneumoniae type 3. Twenty experiments conducted in an 8-l lab-scale fermentor were designed to assess fermentation pH, supplemented glucose concentration, and stirring rate. The predicted highest CPS production by the obtained optimization model equation was 256.14 mg/l at optimal conditions [pH 7.5, stirring rate 180 rpm, and supplemented glucose concentration 1% (w/v)]. The validity of the response model was confirmed by the good agreement between the predicted and experimental results. The maximum amount of CPS obtained was 255.03±2.23 mg/l.
|
|
|
KEYWORD
|
|
|
Capsular polysaccharide, optimization, response surface methodology, Streptococcus pneumoniae, vaccine
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|